MTHFD1 Polyclonal Antibody
As low as $166.00
In stock
Only %1 left
Code:
144-08661
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Product Description

Specifications

Size 50 ul, 100 ul, 200 ul
Species Human, Mouse, Rat
Host Species Rabbit
Clonality Polyclonal
Isotype Igg
Buffers 50% glycerol, PBS with 0.02% sodium azide, pH7.3.
Protein Name / Synonyms MTHFD1, MTHFC, MTHFD, C-1-tetrahydrofolate synthase, cytoplasmic
Accession Number P11586
Gene Symbols MTHFD1
Gene Id 4522
Purity Affinity purification

Background

This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain.

Dilutions

WB 1:200 - 1:2000

Format

Calculated MW
101kDa
Observed MW
115kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human MTHFD1 (NP_005947.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using MTHFD1 antibody (144-08661) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - MTHFD1 antibody (144-08661)

Expiration:
12 months from the date of shipment when stored properly.
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