Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Two transcript variants have been identified for this gene.
WB 1:500 - 1:2000
IHC 1:50 - 1:200
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human MYL1 (NP_524146.1).
Western blot analysis of extracts of various cell lines, using MYL1 antibody (144-08438) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human prostate using MYL1 antibody (144-08438) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using MYL1 antibody (144-08438) at dilution of 1:100 (40x lens).
12 months from the date of shipment when stored properly.