MYO5A Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9Y4I1 |
| Gene Id | 4644 |
| Gene Symbols | MYO5A |
| Protein Name / Synonyms | MYO5A, GS1, MYH12, MYO5, MYR12, myosin VA |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. The protein encoded by this gene is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1), Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease, or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported, but the full-length nature of some variants has not been determined.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
212kDa/215kDa/218kDa
Observed MW
215kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 950-1150 of human MYO5A (NP_000250.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using MYO5A antibody (144-09830) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat kidney using MYO5A antibody (144-09830) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using MYO5A antibody (144-09830) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate cancer using MYO5A antibody (144-09830) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human rectum using MYO5A antibody (144-09830) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.