NASP Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P49321 |
| Gene Id | 4678 |
| Gene Symbols | NASP |
| Protein Name / Synonyms | NASP, FLB7527, HMDRA1, PRO1999, nuclear autoantigenic sperm protein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
48kDa/78kDa/85kDa/86kDa
Observed MW
85kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 180-449 of human NASP (NP_689511.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using NASP antibody (144-06938) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human breast cancer using NASP Antibody (144-06938) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using NASP Antibody (144-06938) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using NASP Antibody (144-06938) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using NASP Antibody (144-06938) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat stomach using NASP Antibody (144-06938) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using NASP Antibody (144-06938) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendicitis using NASP Antibody (144-06938) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human brain astrocytoma using NASP Antibody (144-06938) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using NASP Antibody (144-06938) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using NASP Antibody (144-06938) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using NASP Antibody (144-06938) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using NASP Antibody (144-06938) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using NASP Antibody (144-06938) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human adenomyosis using NASP Antibody (144-06938) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using NASP Antibody (144-06938) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using NASP Antibody (144-06938) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using NASP Antibody (144-06938) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using NASP Antibody (144-06938) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.