This gene, isolated using the differential display method to detect tissue-specific genes, is specifically expressed in kidney and liver. The encoded protein shows amino acid sequence similarity to N-acetyltransferases. A similar protein in Xenopus affects cell adhesion and gastrulation movements, and may be localized in the secretory pathway. A highly similar paralog is found in a cluster with this gene.
Dilutions
WB 1:500 - 1:2000 IHC 1:50 - 1:100
Format
Calculated MW
25kDa
Observed MW
26kDa
Immunogen
Recombinant protein of human NAT8
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using NAT8 antibody (144-07759) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse brain using NAT8 antibody (144-07759) (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using NAT8 antibody (144-07759) (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using NAT8 antibody (144-07759) (40x lens).
Expiration:
12 months from the date of shipment when stored properly.
