[KO Validated] NDUFB4 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O95168 |
| Gene Id | 4710 |
| Gene Symbols | NDUFB4 |
| Protein Name / Synonyms | NDUFB4, B15, CI-B15, NADH:ubiquinone oxidoreductase subunit B4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a non-catalytic subunit of the multisubunit NADH:ubiquinone oxidoreductase, the first enzyme complex in the mitochondrial electron transport chain (complex I). Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to ubiquinone.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
14kDa/15kDa
Observed MW
15kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human NDUFB4 (NP_001161803.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using NDUFB4 antibody (144-60491) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.![Western blot - [KO Validated] NDUFB4 Polyclonal Antibody (144-60491)](https://cdn.sanity.io/images/g51potcz/production/fcebc6bb5d98a7c6861fb91697695f2ba3e92f33-469x350.jpg)
Western blot analysis of extracts from normal (control) and NDUFB4 knockout (KO) HeLa cells, using NDUFB4 antibody (144-60491) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.![Western blot - [KO Validated] NDUFB4 Polyclonal Antibody (144-60491)](https://www.raybiotech.com/files/images/Antibodies/144/144-60491/2.jpg)
Expiration:
12 months from the date of shipment when stored properly.