NDUFS3 Polyclonal Antibody
As low as $234.00
In stock
Only %1 left
Code:
144-08013
-
+

Product Description

Specifications

Size 50 ul, 100 ul, 200 ul
Species Human, Mouse, Rat
Host Species Rabbit
Clonality Polyclonal
Isotype Igg
Buffers 50% glycerol, PBS with 0.02% sodium azide, pH7.3.
Protein Name / Synonyms NDUFS3, CI-30, NADH:ubiquinone oxidoreductase core subunit S3
Accession Number O75489
Gene Symbols NDUFS3
Gene Id 4722
Purity Affinity purification

Background

This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW
14kDa/30kDa
Observed MW
30kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 37-264 of human NDUFS3 (NP_004542.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using NDUFS3 antibody (144-08013) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] NDUFS3 Polyclonal Antibody (144-08013)

Western blot analysis of extracts from normal (control) and NDUFS3 knockout (KO) 293T cells, using NDUFS3 antibody (144-08013) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - [KO Validated] NDUFS3 Polyclonal Antibody (144-08013)

Immunohistochemistry of paraffin-embedded human liver injury using NDUFS3 antibody (144-08013) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] NDUFS3 Polyclonal Antibody (144-08013)

Immunohistochemistry of paraffin-embedded rat brain using NDUFS3 antibody (144-08013) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] NDUFS3 Polyclonal Antibody (144-08013)

Expiration:
12 months from the date of shipment when stored properly.
Write Your Own Review
Your Rating
Submit a more detailed review for this product on Biocompare.com to receive an additional $20 Amazon gift card!

Ask a Question

Display name:
Your location:*