This gene encodes one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported.
Recombinant fusion protein containing a sequence corresponding to amino acids 1-287 of human NIN (NP_891991.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of Jurkat cells, using NIN antibody (144-08215) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using NIN antibody (144-08215) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using NIN antibody (144-08215) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using NIN antibody (144-08215) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using NIN antibody (144-08215) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.
