NME1 Polyclonal Antibody
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P15531 |
| Gene Id | 4830 |
| Gene Symbols | NME1 |
| Protein Name / Synonyms | NME1, AWD, GAAD, NB, NBS, NDKA, NDPK-A, NDPKA, NM23, NM23-H1, nucleoside diphosphate kinase A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
17kDa/19kDa/30kDa
Observed MW
21kDa
Immunogen
A synthetic peptide of human NME1
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using NME1 antibody (144-00259) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat heart using NME1 Antibody (144-00259) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using NME1 Antibody (144-00259) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of A549 cells using NME1 antibody (144-00259). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.