Phospho-eNOS-S1177 pAb
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P29474 |
| Gene Id | 4846 |
| Gene Symbols | NOS3 |
| Protein Name / Synonyms | NOS3, ECNOS, eNOS, nitric oxide synthase 3 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
67kDa/68kDa/133kDa
Observed MW
133kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding S1177 of human eNOS
Modification
Phospho S1177
Storage
-20°C
Images
Western blot analysis of extracts of SKOV3 cells, using Phospho-eNOS-S1177 antibody (144-61502). SKOV3 cells were treated by EGF.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Western blot analysis of extracts of NIH/3T3 cells,using Phospho-eNOS-S1177 antibody (144-61502) at 1:1000 dilution. NIH/3T3 cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Expiration:
12 months from the date of shipment when stored properly.