[KO Validated] NUDT1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P36639 |
| Gene Id | 4521 |
| Gene Symbols | NUDT1 |
| Protein Name / Synonyms | NUDT1, MTH1, nudix hydrolase 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:20 - 1:50
IHC 1:50 - 1:200
IP 1:20 - 1:50
Format
Calculated MW
17kDa/19kDa/20kDa/22kDa
Observed MW
18kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human NUDT1 (NP_945192.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using NUDT1 antibody (144-60285) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.![Western blot - [KO Validated] NUDT1 Polyclonal Antibody (144-60285)](https://cdn.sanity.io/images/g51potcz/production/ec611a3551f8d7ea2330ac3db5f3b7aab80f0b59-386x350.jpg)
Western blot analysis of extracts from normal (control) and NUDT1 knockout (KO) 293T cells, using NUDT1 antibody (144-60285) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.![Western blot - [KO Validated] NUDT1 Polyclonal Antibody (144-60285)](https://cdn.sanity.io/images/g51potcz/production/059ffbce5a537a2a07fbf9a002a180b2ebf480e9-350x350.jpg)
Immunohistochemistry of paraffin-embedded human gastric cancer using NUDT1 antibody (144-60285) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] NUDT1 Polyclonal Antibody (144-60285)](https://cdn.sanity.io/images/g51potcz/production/d0704b5f79229eea25bf4fde23e75411a06519d4-317x350.jpg)
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug NUDT1 antibody (144-60285). Western blot was performed from the immunoprecipitate using NUDT1 antibody (144-60285) at a dilition of 1:1000.![Immunoprecipitation - [KO Validated] NUDT1 Polyclonal Antibody (144-60285)](https://www.raybiotech.com/files/images/Antibodies/144/144-60285/4.jpg)
Expiration:
12 months from the date of shipment when stored properly.