OTC Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P00480 |
| Gene Id | 5009 |
| Gene Symbols | OTC |
| Protein Name / Synonyms | OTC, OCTD, ornithine carbamoyltransferase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This nuclear gene encodes a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also.
Dilutions
WB 1:1000 - 1:4000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
39kDa
Observed MW
40kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 215-354 of human OTC (NP_000522.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using OTC antibody (144-09834) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human stomach using OTC antibody (144-09834) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using OTC antibody (144-09834) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using OTC antibody (144-09834) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.