Synonyms
FLJ20113 antibody, FLJ40710 antibody, MGC111158 antibody, MGC4584 antibody, OTB1 antibody, OTU1 antibody, OTUB1 antibody, otubain-1 antibody, ubiquitin thioesterase OTUB1 antibody, deubiquitinating enzyme OTUB1 antibody, ubiquitin-specific protease otubain 1 antibody, OTU domain-containing ubiquitin aldehyde-binding protein 1 antibody, ubiquitin-specific-processing protease OTUB1 antibody, OTU-domain Ubal-binding 1 antibody, "OTU domain, ubiquitin aldehyde binding 1 antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
31 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 149 of OTUB1 (Uniprot ID# Q96FW1)
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed HeLa, using OTUB1(107-10304) antibody at 1:50 dilution.

Sample (50 ug of whole cell lysate)
A: Mouse liver
12% SDS PAGE
107-10304 diluted at 1:500

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2
12% SDS PAGE
107-10304 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.