OXCT1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P55809 |
| Gene Id | 5019 |
| Gene Symbols | OXCT1 |
| Protein Name / Synonyms | OXCT1, OXCT, SCOT, 3-oxoacid CoA-transferase 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a member of the 3-oxoacid CoA-transferase gene family. The encoded protein is a homodimeric mitochondrial matrix enzyme that plays a central role in extrahepatic ketone body catabolism by catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate. Mutations in this gene are associated with succinyl CoA:3-oxoacid CoA transferase deficiency.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
13kDa/56kDa
Observed MW
56kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 261-520 of human OXCT1 (NP_000427.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using OXCT1 antibody (144-08139) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.![Western blot - [KO Validated] OXCT1 Polyclonal Antibody (144-08139)](https://cdn.sanity.io/images/g51potcz/production/6c6ac91ac7cf5f90cd3aa615b3619cec72cfd3f1-613x350.jpg)
Western blot analysis of extracts from normal (control) and OXCT1 knockout (KO) HeLa cells, using OXCT1 antibody (144-08139) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.![Western blot - [KO Validated] OXCT1 Polyclonal Antibody (144-08139)](https://cdn.sanity.io/images/g51potcz/production/7a4161ae6527c4f26c4d5ec6e9632157a318742e-350x350.jpg)
Immunohistochemistry of paraffin-embedded human esophagus using OXCT1 antibody (144-08139) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] OXCT1 Polyclonal Antibody (144-08139)](https://cdn.sanity.io/images/g51potcz/production/184cbdee8ddd744df57357c991704aeaf9741dd7-350x350.jpg)
Immunohistochemistry of paraffin-embedded human stomach using OXCT1 antibody (144-08139) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] OXCT1 Polyclonal Antibody (144-08139)](https://www.raybiotech.com/files/images/Antibodies/144/144-08139/4.jpg)
Expiration:
12 months from the date of shipment when stored properly.