PARK7 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q99497 |
| Gene Id | 11315 |
| Gene Symbols | PARK7 |
| Protein Name / Synonyms | PARK7, DJ-1, DJ1, GATD2, HEL-S-67p, protein DJ-1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Research Area | Inflammation, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
19kDa
Observed MW
20kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-189 of human PARK7 (NP_001116849.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PARK7 antibody (144-00987) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.![Western blot - [KO Validated] PARK7 Polyclonal Antibody (144-00987)](https://cdn.sanity.io/images/g51potcz/production/3c8c261b198dfe10aab053a9c89a4003018268ef-287x350.jpg)
Western blot analysis of extracts from normal (control) and PARK7 knockout (KO) 293T cells, using PARK7 antibody (144-00987) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.![Western blot - [KO Validated] PARK7 Polyclonal Antibody (144-00987)](https://cdn.sanity.io/images/g51potcz/production/3d57f9d7e96a2bcc3886304139edb804e2d5756b-367x550.jpg)
Immunofluorescence analysis of HeLa cells using PARK7 antibody (144-00987). Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] PARK7 Polyclonal Antibody (144-00987)](https://www.raybiotech.com/files/images/Antibodies/144/144-00987/3.jpg)
Expiration:
12 months from the date of shipment when stored properly.