PAWR Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q96IZ0 |
| Gene Id | 5074 |
| Gene Symbols | PAWR |
| Protein Name / Synonyms | PAWR, PAR4, Par-4, pro-apoptotic WT1 regulator |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The tumor suppressor WT1 represses and activates transcription. The protein encoded by this gene is a WT1-interacting protein that itself functions as a transcriptional repressor. It contains a putative leucine zipper domain which interacts with the zinc finger DNA binding domain of WT1. This protein is specifically upregulated during apoptosis of prostate cells.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
36kDa
Observed MW
43kDa
Immunogen
Recombinant protein of human PAWR
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PAWR antibody (144-03063) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat pancreas using PAWR Antibody (144-03063) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PAWR Antibody (144-03063) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PAWR Antibody (144-03063) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PAWR Antibody (144-03063) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.