Rabbit Anti-PCK1 (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P35558 |
| Gene Id | 5105 |
| Gene Symbols | PCK1 |
| Protein Name / Synonyms | PCK1 (C-term) |
| Clonality | Polyclonal |
| Preparation | This PCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 592-622 amino acids from the C-terminal region of human PCK1. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme of the encoded protein also has been characterized.
Specificity
Other Names
PCK1; PEPCK1; Phosphoenolpyruvate carboxykinase, cytosolic [GTP]; Phosphoenolpyruvate carboxylase
NCBI Accession #
NP_002582.3
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
69195
Recommended Dilutions
WB: 1:1000; IF: 1:10-50; IHC: 1:50-100
 | Western blot analysis of PCK1 Antibody (C-term) (Cat# 102-17797) in rat primary hepatocyte cell line lysates. PCK1 (arrow) was detected using the purified Pab. |
 | PCK1 Antibody (C-term) (Cat# 102-17797) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PCK1 antibody detected the PCK1 protein (arrow). |
 | Confocal immunofluorescent analysis of PCK1 Antibody (C-term)(Cat# 102-17797) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue). |
 | The anti-PCK1 Pab (Cat# 102-17797) is used in Western blot to detect PCK1 in mouse liver (Lane 1) and mouse small intestine tissue lysates (Lane 2). |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.