PCK2 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q16822 |
| Gene Id | 5106 |
| Gene Symbols | PCK2 |
| Protein Name / Synonyms | PCK2, PEPCK, PEPCK-M, PEPCK2, phosphoenolpyruvate carboxykinase 2, mitochondrial |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IP |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IP 1:20 - 1:100
IHC 1:50 - 1:100
IP 1:20 - 1:100
Format
Calculated MW
47kDa/70kDa
Observed MW
71kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PCK2 (NP_004554.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PCK2 antibody (144-08446) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 10s.
Immunoprecipitation analysis of extracts of HepG2 cells using PCK2 antibody (144-08446). Western blot was performed from the immunoprecipitate using PCK2 antibody (144-08446) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.