The protein encoded by this gene serves to anchor phosphodiesterase 4D to the Golgi/centrosome region of the cell. Defects in this gene may be a cause of myeloproliferative disorder (MBD) associated with eosinophilia. Several transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000 IHC 1:50 - 1:200
Format
Calculated MW
20-36kDa/112-128kDa/254-267kDa
Observed MW
265kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human PDE4DIP (NP_071754.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of mouse skeletal muscle, using PDE4DIP antibody (144-07765) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat heart using PDE4DIP antibody (144-07765) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using PDE4DIP antibody (144-07765) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using PDE4DIP antibody (144-07765) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.
