PDIA6 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q15084 |
| Gene Id | 10130 |
| Gene Symbols | PDIA6 |
| Protein Name / Synonyms | PDIA6, ERP5, P5, TXNDC7, protein disulfide-isomerase A6 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, a TRX-like domain, and a C-terminal ER-retention sequence. This protein inhibits the aggregation of misfolded proteins and exhibits both isomerase and chaperone activity. Alternative splicing results in multiple transcript variants encoding different isoforms.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
47kDa/48kDa/53kDa
Observed MW
48kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human PDIA6 (NP_005733.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PDIA6 antibody (144-07055) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using PDIA6 Antibody (144-07055) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PDIA6 Antibody (144-07055) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using PDIA6 antibody (144-07055). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.