The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH. The protein encoded by this gene is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000 IHC 1:50 - 1:100
Format
Calculated MW
46kDa/48kDa
Observed MW
47kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 137-406 of human PDK3 (NP_005382.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PDK3 antibody (144-08028) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded mouse lung using PDK3 antibody (144-08028) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PDK3 antibody (144-08028) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using PDK3 antibody (144-08028) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using PDK3 antibody (144-08028) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.
