PECAM1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P16284 |
| Gene Id | 18613 |
| Gene Symbols | PECAM1 |
| Protein Name / Synonyms | PECAM1, CD31, CD31/EndoCAM, GPIIA', PECA1, PECAM-1, endoCAM, platelet endothelial cell adhesion molecule |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.05% proclin300, 50% glycerol, pH 7.3 |
| Recommended Applications | Western Blotting, IF |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
79kDa/80kDa/81kDa/82kDa
Observed MW
130kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of mouse PECAM1 (NP_001292086.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of mouse liver, using PECAM1 antibody (144-60020) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunofluorescence analysis of U2OS cells using PECAM1 antibody (144-60020) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.