This gene encodes a protein containing a PHD (plant homeodomain) type zinc finger. This gene has been identified in some studies as a candidate gene for asthma. Naturally-occurring readthrough transcription may occur from the upstream SETDB2 (SET domain bifurcated 2) gene to this locus. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000 IF 1:50 - 1:100
Format
Calculated MW
33kDa/37kDa
Observed MW
34kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human PHF11 (NP_001035533.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PHF11 antibody (144-07803) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunofluorescence analysis of A549 cells using PHF11 antibody (144-07803). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.
