Anti-Phospho-AKT1-T308 Antibody

Phospho-AKT1-T308 Polyclonal Antibody
As low as $211.00
In stock
Only %1 left

Product Description


Size 50 ul, 100 ul, 200 ul
Species Human, Mouse
Host Species Rabbit
Isotype Igg
Buffers 50% glycerol, PBS with 0.02% sodium azide, pH7.3.
Protein Name / Synonyms AKT1, AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, AKT serine/threonine kinase 1
Accession Number P31749
Gene Symbols AKT1
Gene Id 207
Purity Affinity purification


The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.


WB 1:500 - 1:2000
IF 1:50 - 1:200


Calculated MW
Observed MW


A synthetic phosphorylated peptide around T308 of human AKT1 (NP_005154.2).


Phospho T308




Western blot analysis of extracts of HeLa cells, using Phospho-AKT1-T308 antibody (144-50304) at 1:2000 dilution. HeLa cells were treated by 10% FBS for after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - Phospho-AKT1-T308 pAb (144-50304)

Immunofluorescence analysis of HeLa cells using Phospho-AKT1-T308 antibody (144-50304) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - Phospho-AKT1-T308 pAb (144-50304)

12 months from the date of shipment when stored properly.
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