This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.
A synthetic phosphorylated peptide around S1423 of human BRCA1 (NP_009225.1).
Modification
Phospho S1423
Storage
-20°C
Images
Western blot analysis of extracts of HT-29 cells,using Phospho-BRCA1-S1423 antibody (144-50232) at 1:2000 dilution. HT-29 cells were treated by Anisomycin (5ug/ml) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat liver using Phospho-BRCA1-S1423 antibody (144-50232) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.
