Phospho-CDK1-T14 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P06493 |
| Gene Id | 983 |
| Gene Symbols | CDK1 |
| Protein Name / Synonyms | CDK1, CDC2, CDC28A, P34CDC2, cyclin-dependent kinase 1 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:1000
IF 1:50 - 1:100
IF 1:50 - 1:100
Format
Calculated MW
27kDa/34kDa
Observed MW
34kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding T14 of human CDK1
Modification
Phospho T14
Storage
-20°C
Images
Western blot analysis of extracts of HT-29 and HeLa cells, using Phospho-CDK1-T14 antibody (144-50015) at 1:1000 dilution. HT-29 cells were treated by serum-starvation overnight. HeLa cells were treated by UV for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 10min.
Immunofluorescence analysis of MCF-7 cells using Phospho-CDK1-T14 antibody (144-50015). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.