Anti-Phospho-CDK1-T161 Antibody

Phospho-CDK1-T161 Polyclonal Antibody
As low as $211.00
In stock
Only %1 left

Product Description


Size 50 ul, 100 ul, 200 ul
Species Human
Host Species Rabbit
Isotype Igg
Buffers 50% glycerol, PBS with 0.02% sodium azide, pH7.3.
Protein Name / Synonyms CDK1, CDC2, CDC28A, P34CDC2, cyclin-dependent kinase 1
Accession Number P06493
Gene Symbols CDK1
Gene Id 983
Concentration (lot specific) 0.75 mg/ml
Purity Affinity purification


The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.


WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100


Calculated MW
Observed MW


A phospho specific peptide corresponding to residues surrounding T161 of human CDK1


Phospho T161




Western blot analysis of extracts of HeLa cells, using Phospho-CDK1-T161 antibody (144-50324) at 1:1000 dilution. HeLa cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - Phospho-CDK1-T161 pAb (144-50324)

Immunofluorescence analysis of MCF-7 cells using Phospho-CDK1-T161 antibody (144-50324). Blue: DAPI for nuclear staining.
Immunofluorescence - Phospho-CDK1-T161 pAb (144-50324)

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-CDK1-T161 pAb (144-50324). Western blot was performed from the immunoprecipitate using Phospho-CDK1-T161 pAb (144-50324) at a dilition of 1:1000. Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37? for 2 minutes after serum-starvation overnight.
Immunoprecipitation - Phospho-CDK1-T161 pAb (144-50324)

12 months from the date of shipment when stored properly.
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