The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100
Format
Calculated MW
27kDa/34kDa
Observed MW
34kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding T161 of human CDK1
Modification
Phospho T161
Storage
-20°C
Images
Western blot analysis of extracts of HeLa cells, using Phospho-CDK1-T161 antibody (144-50324) at 1:1000 dilution. HeLa cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.
Immunofluorescence analysis of MCF-7 cells using Phospho-CDK1-T161 antibody (144-50324). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-CDK1-T161 pAb (144-50324). Western blot was performed from the immunoprecipitate using Phospho-CDK1-T161 pAb (144-50324) at a dilition of 1:1000. Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37? for 2 minutes after serum-starvation overnight.
Expiration:
12 months from the date of shipment when stored properly.
