Phospho-ERK3-S189 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q16659 |
| Gene Id | 5597 |
| Gene Symbols | MAPK6 |
| Protein Name / Synonyms | MAPK6, ERK3, HsT17250, PRKM6, p97MAPK, mitogen-activated protein kinase 6 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
82kDa
Observed MW
120kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding S189 of human ERK3
Modification
Phospho S189
Storage
-20°C
Images
Western blot analysis of extracts of 293 cells, using Phospho-ERK3-S189 antibody (144-50051) at 1:1000 dilution. 293 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Expiration:
12 months from the date of shipment when stored properly.