This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
54kDa/60kDa
Observed MW
61kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding S21 of human FYN
Modification
Phospho S21
Storage
-20°C
Images
Western blot analysis of extracts of A-431 cells, using Phospho-FYN-S21 antibody (144-50510) at 1:1000 dilution. HL-60 cells were treated by 10% FBS after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Expiration:
12 months from the date of shipment when stored properly.
