Phospho-GSK3A-S21 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P49840 |
| Gene Id | 2931 |
| Gene Symbols | GSK3A |
| Protein Name / Synonyms | GSK3A, glycogen synthase kinase-3 alpha |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IP |
| Research Area | Cardiovascular Disease, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:100
IHC 1:50 - 1:200
IP 1:50 - 1:100
Format
Calculated MW
50kDa
Observed MW
51kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding S21 of human GSK3A
Modification
Phospho S21
Storage
-20°C
Images
Western blot analysis of extracts of 293 and HeLa cells, using Phospho-GSK3A-S21 antibody (144-50582) at 1:1000 dilution. 293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. HeLa cells were treated by Insulin (0.01U/ml) for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-GSK3A-S21 pAb (144-50582). Western blot was performed from the immunoprecipitate using Phospho-GSK3A-S21 pAb (144-50582) at a dilition of 1:1000. 293T cells were treated by Insulin (100 nM) at 37? for 10 minutes after serum-starvation overnight.
Expiration:
12 months from the date of shipment when stored properly.