This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease.
A phospho specific peptide corresponding to residues surrounding S21 of human GSK3A
Modification
Phospho S21
Storage
-20°C
Images
Western blot analysis of extracts of 293 and HeLa cells, using Phospho-GSK3A-S21 antibody (144-50582) at 1:1000 dilution. 293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. HeLa cells were treated by Insulin (0.01U/ml) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-GSK3A-S21 pAb (144-50582). Western blot was performed from the immunoprecipitate using Phospho-GSK3A-S21 pAb (144-50582) at a dilition of 1:1000. 293T cells were treated by Insulin (100 nM) at 37? for 10 minutes after serum-starvation overnight.
Expiration:
12 months from the date of shipment when stored properly.
