Phospho-H2AFX-S139 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P16104 |
| Gene Id | 3014 |
| Gene Symbols | H2AFX |
| Protein Name / Synonyms | H2AFX, H2A.X, H2A/X, H2AX, histone H2AX |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IHC 1:50 - 1:100
IF 1:50 - 1:100
Format
Calculated MW
15kDa
Observed MW
17kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding S139 of human Histone H2A.x
Modification
Phospho S139
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using Phospho-H2A.X-S139 antibody (144-50099) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) for 5 hours. NIH/3T3 cells were treated by UV for 15-30 minutes. C6 cells were treated by UV for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human gastric cancer using Phospho-Histone H2AFX-S139 antibody (144-50099) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using Phospho-Histone H2AFX-S139 antibody (144-50099) at dilution of 1:200 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.