Anti-Phospho-HSPB1-S15 Antibody

Phospho-HSPB1-S15 Polyclonal Antibody
As low as $211.00
In stock
Only %1 left

Product Description


Size 50 ul, 100 ul, 200 ul
Species Human, Mouse, Rat
Host Species Rabbit
Isotype Igg
Buffers 50% glycerol, PBS with 0.02% sodium azide, pH7.3.
Protein Name / Synonyms HSPB1, CMT2F, HEL-S-102, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27, heat shock protein beta-1
Accession Number P04792
Gene Symbols HSPB1
Gene Id 3315
Purity Affinity purification


The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).


WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:100


Calculated MW
Observed MW


A phospho specific peptide corresponding to residues surrounding S15 of human HSPB1


Phospho S15




Western blot analysis of extracts of HeLa cells, using Phospho-HSPB1-S15 antibody (144-50040) at 1:1000 dilution. HeLa cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 5min.
Western blot - Phospho-HSPB1-S15 pAb (144-50040)

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-HSPB1-S15 pAb (144-50040). Western blot was performed from the immunoprecipitate using Phospho-HSPB1-S15 pAb (144-50040) at a dilition of 1:1000. HeLa cells were treated by EGF (100 ng/ml) at 37? for 30 minutes after serum-starvation overnight.
Immunoprecipitation - Phospho-HSPB1-S15 pAb (144-50040)

12 months from the date of shipment when stored properly.
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