Phospho-HSPB1-S15 Polyclonal Antibody
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P04792 |
| Gene Id | 3315 |
| Gene Symbols | HSPB1 |
| Protein Name / Synonyms | HSPB1, CMT2F, HEL-S-102, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27, heat shock protein beta-1 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IP |
| Research Area | Angiogenesis, Cancer, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:100
IHC 1:50 - 1:200
IP 1:50 - 1:100
Format
Calculated MW
22kDa
Observed MW
28kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding S15 of human HSPB1
Modification
Phospho S15
Storage
-20°C
Images
Western blot analysis of extracts of HeLa cells, using Phospho-HSPB1-S15 antibody (144-50040) at 1:1000 dilution. HeLa cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 5min.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-HSPB1-S15 pAb (144-50040). Western blot was performed from the immunoprecipitate using Phospho-HSPB1-S15 pAb (144-50040) at a dilition of 1:1000. HeLa cells were treated by EGF (100 ng/ml) at 37? for 30 minutes after serum-starvation overnight.
Expiration:
12 months from the date of shipment when stored properly.