Phospho-INSR-Y1150/IGF1R-Y1135 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P08069|P06213 |
| Gene Id | 3480|3643 |
| Gene Symbols | IGF1R, INSR |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
154kDa/155kDa/156kDa
Observed MW
80kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding Y1150 of human INSR and Y1135 of human IGF1R
Modification
Phospho Y1150/Y1135
Storage
-20°C
Images
Western blot analysis of extracts of 293 and HepG2 cells, using Phospho-INSR-Y1150/IGF1R-Y1135 antibody (144-50044) at 1:1000 dilution. 293 cells were treated by EGF (25ug/ml) for 30 minutes after serum-starvation overnight. HepG2 cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Expiration:
12 months from the date of shipment when stored properly.