The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
53kDa/56kDa/64kDa/67kDa
Observed MW
67kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding S412 of human MAP3K7
Modification
Phospho S412
Storage
-20°C
Images
Western blot analysis of extracts of K-562 cells, using Phospho-MAP3K7-S412 antibody (144-50071) at 1:1000 dilution. K562 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight or treated by PMA/TPA (200nM). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Expiration:
12 months from the date of shipment when stored properly.
