The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.
Dilutions
WB 1:500 - 1:2000 IF 1:50 - 1:200
Format
Calculated MW
288kDa
Observed MW
289kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding serine 2448 of human mTOR/FRAP
Modification
Phospho S2448
Storage
-20°C
Images
Western blot analysis of extracts of NIH/3T3 and C6 cells, using Phospho-MTOR-S2448 antibody (144-50094) at 1:2000 dilution. NIH/3T3 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. C6 cells were treated by IGF-1 (50 ng/ml) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30S.
Immunofluorescence analysis of HeLa cells using Phospho-MTOR-S2448 antibody (144-50094) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.
