This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Dilutions
WB 1:500 - 1:2000 IP 1:50 - 1:100
Format
Calculated MW
72kDa/81kDa/82kDa/83kDa
Observed MW
90kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding S380 of human P90RSK
Modification
Phospho S380
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using Phospho-P90RSK-S380 antibody (144-50562) at 1:1000 dilution or RPS6KA1 antibody (144-015718). HeLa cells were treated by PMA/TPA (200 nM) at 37? for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using Phospho-P90RSK-S380 antibody (144-50562) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37? for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-P90RSK-S380 pAb (144-50562). Western blot was performed from the immunoprecipitate using Phospho-P90RSK-S380 pAb (144-50562) at a dilition of 1:1000. HeLa cells were treated by EGF (100 ng/ml) at 37? for 30 minutes after serum-starvation overnight.
Expiration:
12 months from the date of shipment when stored properly.
