This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
24kDa/82kDa/122kDa
Observed MW
180kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding Y849 of human PDGFRA
Modification
Phospho Y849
Storage
-20°C
Images
Western blot analysis of extracts of HepG2 cells, using Phospho-PDGFRA-Y849 antibody (144-50569) at 1:1000 dilution. HepG2 cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Expiration:
12 months from the date of shipment when stored properly.
