WB 1:500 - 1:2000
A phospho specific peptide corresponding to residues surrounding S643/676 of human PKC?/?
Western blot analysis of extracts of RAW264.7 cells, using Phospho-PKC?/?-S643/676 antibody (144-50567) at 1:1000 dilution. RAW 264.7 cells were treated by PMA (200 nM) for 30 minutes .
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 5s.
12 months from the date of shipment when stored properly.