Phospho-Rb-S811 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P06400 |
| Gene Id | 5925 |
| Gene Symbols | RB1 |
| Protein Name / Synonyms | RB1, OSRC, PPP1R130, RB, p105-Rb, pRb, pp110, RB transcriptional corepressor 1 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Research Area | Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.
Dilutions
WB 1:1000 - 1:2000
IF 1:50 - 1:100
IF 1:50 - 1:100
Format
Calculated MW
106kDa
Observed MW
106kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding S811 of human RB
Modification
Phospho S811
Storage
-20°C
Images
Western blot analysis of extracts of 293 and HeLa cells, using Phospho-Rb-S811 antibody (144-50089) at 1:1000 dilution. HeLa cells were treated by UV for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Immunofluorescence analysis of U2OS cells using Phospho-Rb-S811 antibody (144-50089). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.