Phospho-SMC1A-S957 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q14683 |
| Gene Id | 8243 |
| Gene Symbols | SMC1A |
| Protein Name / Synonyms | SMC1A, CDLS2, DXS423E, SB1.8, SMC1, SMC1L1, SMC1alpha, SMCB, structural maintenance of chromosomes 1A |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:100
IP 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:20 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
143kDa
Observed MW
160kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding S957 of human SMC1A
Modification
Phospho S957
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using Phospho-SMC1A-S957 antibody (144-50090) at 1:1000 dilution. HeLa and NIH/3T3 cells were treated by UV for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 3s.
Immunofluorescence analysis of U2OS cells using Phospho-SMC1A-S957 antibody (144-50090). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using Phospho-SMC1A-S957 antibody (144-50090). Green?GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-SMC1A-S957 pAb (144-50090). Western blot was performed from the immunoprecipitate using Phospho-SMC1A-S957 pAb (144-50090) at a dilition of 1:1000. HeLa cells were treated by UV at room temperature for 15-30 minutes.
Expiration:
12 months from the date of shipment when stored properly.