Phospho-Syk-Y352/ZAP70-Y319 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P43403|P43405 |
| Gene Id | 7535|6850 |
| Gene Symbols | SYK, ZAP70 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
69kDa/72kDa/35kDa/55kDa
Observed MW
72kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding Y352 of human Syk and Y319 of human ZAP70
Modification
Phospho Y352/Y319
Storage
-20°C
Images
Western blot analysis of extracts of Jurkat cells, using Phospho-Syk-Y352/ZAP70-Y319 antibody (144-50501) at 1:1000 dilution. Jurkat cells were treated by 10% FBS for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Expiration:
12 months from the date of shipment when stored properly.