This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
WB 1:500 - 1:2000
IP 1:50 - 1:100
A phospho specific peptide corresponding to residues surrounding Y525/526 of human Syk
Western blot analysis of extracts of Jurkat cells, using Phospho-Syk-Y525/526 antibody (144-50542) at 1:1000 dilution. Jurkat cells were treated by 10% FBS for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
12 months from the date of shipment when stored properly.