PHOX2B, NBLST2, NBPhox, PMX2B, paired like homeobox 2b
Accession Number
Q99453
Gene Symbols
PHOX2B
Gene Id
8929
Recommended Applications
Western Blotting,IHC
Purity
Affinity purification
Background
The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription factor involved in the development of several major noradrenergic neuron populations and the determination of neurotransmitter phenotype. The gene product is linked to enhancement of second messenger-mediated activation of the dopamine beta-hydroylase, c-fos promoters and several enhancers, including cyclic amp-response element and serum-response element. Expansion of a 20 amino acid polyalanine tract in this protein by 5-13 aa has been associated with congenital central hypoventilation syndrome.
Dilutions
WB 1:500 - 1:2000 IHC 1:50 - 1:200
Format
Calculated MW
31kDa
Observed MW
32kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human PHOX2B (NP_003915.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PHOX2B antibody (144-09685) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat liver using PHOX2B antibody (144-09685) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using PHOX2B antibody (144-09685) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.
