PIK3CB Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P42338 |
| Gene Id | 5291 |
| Gene Symbols | PIK3CB |
| Protein Name / Synonyms | PIK3CB, P110BETA, PI3K, PI3KBETA, PIK3C1, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Angiogenesis, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes an isoform of the catalytic subunit of phosphoinositide 3-kinase (PI3K). These kinases are important in signaling pathways involving receptors on the outer membrane of eukaryotic cells and are named for their catalytic subunit. The encoded protein is the catalytic subunit for PI3Kbeta (PI3KB). PI3KB has been shown to be part of the activation pathway in neutrophils which have bound immune complexes at sites of injury or infection. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
122kDa
Observed MW
122kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 771-1070 of human PIK3CB (NP_006210.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PIK3CB antibody (144-00982) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse lung using PIK3CB antibody (144-00982) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using PIK3CB antibody (144-00982) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using PIK3CB antibody (144-00982) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.