Rabbit Anti-PIST (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q9HD26 |
| Gene Id | 57120 |
| Gene Symbols | GOPC |
| Protein Name / Synonyms | PIST (C-term) |
| Clonality | Polyclonal |
| Preparation | This PIST antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361-390 amino acids from the C-terminal region of human PIST. |
| Concentration (lot specific) | 1.6 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
PDZ domains contain approximately 90 amino acids and bind the extreme C terminus of proteins in a sequence-specific manner. PIST, a PDZ domain-containing Golgi protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6. Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.
Specificity
Other Names
GOPC; CAL; FIG; Golgi-associated PDZ and coiled-coil motif-containing protein; CFTR-associated ligand; Fused in glioblastoma; PDZ protein interacting specifically with TC10
NCBI Accession #
NP_001017408.1;NP_065132.1
Other Accession #
Q8BH60
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
50520
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50
 |
Western blot analysis of PIST Antibody (C-term) (Cat# 102-17221) in Ramos cell line lysates (35ug/lane). PIST (arrow) was detected using the purified Pab. |
 |
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PIST antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.