PKN1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q16512 |
| Gene Id | 5585 |
| Gene Symbols | PKN1 |
| Protein Name / Synonyms | PKN1, DBK, PAK-1, PAK1, PKN, PKN-ALPHA, PRK1, PRKCL1, protein kinase N1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Dilutions
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
104kDa
Observed MW
120kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PKN1 (NP_002732.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PKN1 antibody (144-00553) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded mouse brain using PKN1 antibody (144-00553) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using PKN1 antibody (144-00553) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using PKN1 antibody (144-00553) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.