WB 1:500 - 1:2000
A synthetic peptide corresponding to a sequence within amino acids 1-54 of human PMAIP1 (NP_066950.1).
Western blot analysis of extracts of various cell lines, using PMAIP1 antibody (144-09801) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
12 months from the date of shipment when stored properly.