PML Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P29590 |
| Gene Id | 5371 |
| Gene Symbols | PML |
| Protein Name / Synonyms | PML, MYL, PP8675, RNF71, TRIM19, protein PML |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Concentration (lot specific) | 4.93 mg/ml |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
47-48kDa/62-97kDa
Observed MW
98kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 300-600 of human PML (NP_150253.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PML antibody (144-01184) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.![Western blot - [KO Validated] PML Polyclonal Antibody (144-01184)](https://cdn.sanity.io/images/g51potcz/production/ebaf7e5fd4242729201ada90938d4d15e036f4c7-370x350.jpg)
Western blot analysis of extracts from normal (control) and PML knockout (KO) HeLa cells, using PML antibody (144-01184) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.![Western blot - [KO Validated] PML Polyclonal Antibody (144-01184)](https://cdn.sanity.io/images/g51potcz/production/73717d1dbe794543104615e4e736d4aa294385a8-467x350.jpg)
Immunohistochemistry of paraffin-embedded human gastric cancer using PML antibody (144-01184) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] PML Polyclonal Antibody (144-01184)](https://cdn.sanity.io/images/g51potcz/production/dc4295577c35fb4ffea7b1c67e37901b87b0c577-467x350.jpg)
Immunohistochemistry of paraffin-embedded human uterine cancer using PML antibody (144-01184) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] PML Polyclonal Antibody (144-01184)](https://cdn.sanity.io/images/g51potcz/production/779b7812aaf68d7b04f2fda03016f3bb22ccbefa-366x550.jpg)
Immunofluorescence analysis of HeLa cells using PML antibody (144-01184). Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] PML Polyclonal Antibody (144-01184)](https://www.raybiotech.com/files/images/Antibodies/144/144-01184/5.jpg)
Expiration:
12 months from the date of shipment when stored properly.