This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described.
WB 1:500 - 1:2000
Recombinant fusion protein containing a sequence corresponding to amino acids 421-575 of human POLL (NP_037406.1).
Western blot analysis of extracts of various cell lines, using POLL antibody (144-08408) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
12 months from the date of shipment when stored properly.