POLR2A Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P24928 |
| Gene Id | 5430 |
| Gene Symbols | POLR2A |
| Protein Name / Synonyms | POLR2A, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1, RNA polymerase II subunit A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
63kDa/217kDa
Observed MW
250kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2A (NP_000928.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using POLR2A antibody (144-011181) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat testis using POLR2A antibody (144-011181) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using POLR2A antibody (144-011181) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using POLR2A antibody (144-011181) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using POLR2A antibody (144-011181) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using POLR2A antibody (144-011181) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse pancreas using POLR2A antibody (144-011181) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.